Amni Sense FAQ

The leaking of amniotic fluid can increase the risk of infection to both mother & baby. Early detection can help to prevent complications or premature delivery, identify a possible rupture after amniocentesis and give a little extra peace of mind.
Amni sense is a simple panty liner  pack for daily use.

 

What is Amni Sense?

Amni sense was originally developed  to clarify whether any wetness felt by expectant mother was the onset of waters breaking or leaking urine.
Amni sense is a panty liner that can detect Amniotic Leakage during pregnancy. It is designed for self-testing and is of value in both normal and high-risk pregnancies.
The test can also helpful to identify whether you may have a vaginal infection, which can be harmful to the baby if left untreated before delivery.

 

When should Amni Sense be used?

Amni Sense should be used from week from 2nd trimister onwards in all normal pregnancies, history of amniotic leak,  after amniocentisis,& whenever there is an unidentified wetness or as recommended by your doctor.

 

Who should use Amni Sense?

Women who have had an amniocentesis should use Amni Sense for 2-3 days afterwards to check that no leakage is occurring. If a positive result is seen then doctor should be consulted right away.
Women having a previous history of pre-term labour or premature rupture of membranes should use Amni Sense daily to monitor themselves.
This means that they can continue their daily routine assured that they are in safe hands. A negative result provides valuable reassurance that any leakage that may occur is going to be quickly detected. A positive test result means that the doctor should be contacted right away.
Amniotic fluid leakage has until now been difficult to detect reliably largely because of urine leakage which is quite common in pregnancy.
Amni Sense can tell the difference between amniotic fluid and urine leakage.
Premature rupture of membranes presents a serious risk to mother and baby - the risk to the mother is infection and pre-term delivery (about 40% of all pre-term deliveries are associated with premature rupture of membranes). The risk to the baby can be very serious. Amniotic fluid leakage occurs in 30-40% of pre-term deliveries and its reliable early detection using Amni Sense will allow women to confidently seek medical assistance promptly.

 

How should Amni Sense be used?

To use Amni Sense, simply attach the special panty liner to your underwear and carry on as normal. When you feel a fluid leak, remove the indicator strip from the panty liner and lay the indicator strip on the white cloth in the open plastic box provided. Close the box. Dispose of the panty liner and after 30 minutes check for a change in colour. If the indicator strip is stained blue or green, amniotic fluid may be leaking from the uterus or you may have a vaginal infection. Under these circumstances you should see your doctor or go to the hospital straight away.
The colour change in cases of amniotic fluid leak is stable for more than 8 hours. If you intend to show the indicating result to your doctor, take the closed plastic box containing the indicator strip to your doctor as soon as possible.
If the indicator strip stains blue or green and then fades back to yellow, or if the colour does not change at all, the fluid leak is probably urine, which is common during pregnancy.

 

What does the Amni Sense kit contain?

The Amni Sense kit contains two test panty liners, two drying tray, usual manual in 8 regional languages for use of Amni sense in proper way

 

Are there any warnings or precautions to be observed when using Amni Sense?

There are only some precautions to observe when using Amni Sense and they are that
you must allow 12 hours after sexual intercourse or vaginal touching before using Amni Sense.
Water interferance may vary the result so keep vaginal area dry during use of Amnisense

Searching For New Method

1) which can done by patient

2) patient friendly

3) Time Saving

4) Help in right management at right time

5) Not need to hospitalized for detection

6) Patient will not feel uncomfortable

7) Quick result

8) Economical

9) Color remains stable for at least 48 hours

10) Kit detects even minimal amounts of Amniotic leaks Clearly distinguishes between amniotic leakage and urine

Drawback of Current Method

• Old methods are either invasive or inaccurate or both .
• Labor intensive
• fern test is neither sensitive nor specific enough for diagnostic determination of premature rupture of membranes.
• pH/nitrazine test is sensitive only when used in women for whom membrane status is known.

CURRENT TECHNIQUE USED FOR AMNIOTIC FLUID DETECTION

Nitrazine (pH)



NITRAZINE TEST



Technique:

Amniotic fluid is alkaline and turns Nitrazine pH indicator blue

Accuracy:

False positive results are up to 17.4%

False negative results are 12.9%

Sensitivity 90.7%

Specificity 77.2%

Drawbacks:

Speculum exam is required to collect the sample. False-positive results may be caused by cervicitis, vaginitis (bacterial vaginosis or Trichomonas), alkaline urine, blood, semen, or antiseptics.

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Ferning

:Technique

Arborization pattern (crystallization) of dry amniotic fluid as seen through a microscope

Accuracy:

False positive results are 5-30%

False negative results are 12.9%

Sensitivity 51.4% (no labor)

Specificity 70.8% (no labor)

Drawbacks:

Speculum exam is required to collect the sample. Requires microscope. False positives may result from contamination of slide with fingerprints or contamination with semen and cervical mucus. False negative may be caused by dry swabs, contamination with blood and discharge. Lack of certified personnel available on the L&D floor to read the slides or the lab’s requirement to have the slides sent down to the lab.

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VAGINAL POOLING

VAGINAL POOLING          

Technique:

Visualization of an amniotic pool in the posterior fornix of the vagina

Accuracy:

Subjective

Drawbacks:

Speculum exam is required. Subjective. Urine, semen, and other fluids can easily be mistaken for amniotic fluid and vice versa.

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Ultrasound

:Technique

Ultrasound can detect oligohydramnios, suggesting loss of amniotic fluid due to membrane rupture

Accuracy:

Not a reliable screening test if used alone. Used only to help confirm diagnosis

Drawbacks:

Time-consuming. Requires equipment and expertise. Can only detect significant loss of amniotic fluid. Can’t confirm cause of fluid loss. Not all hospital facilities have ultrasound expertise easily accessible on a 24/7 basis.

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Amnio-Dye Infusion

Technique:

Instillation of dilute indigo carmine into the amniotic cavity and confirmation of rupture of membranes by documenting leakage of dye into the vagina (staining of tampon) within 20-30 minutes

Accuracy:

"Gold Standard" for diagnosis of rupture of membranes

Drawbacks:

Accurate, but highly invasive (requires amniocentesis). Expensive. Amniocentesis is associated with risk to pregnancy including bleeding, infection, latrogenic rupture of membranes, and loss of pregnancy (approximately 1 in 270).

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AMNISENSE

AMNIOTIC LEAK DETECTION KIT

Sensitivity: 95.76 Specificity:84.46

No invasive procedure require

A single drop of Amniotic fluid is enough for test

Maintain the comfort level of patient and doctors

  



Amniotic fluid Leakage

The amniotic fluid is that fluid surrounding the developing fetus that is found within the amniotic sac contained in the mother's womb.

Physical characteristics ;

pH of is around 7.2.

　

Specific gravity of 1.0069 – 1.008.

　

Volume depends on gestation , 400ml at mid pregnancy and reaches about 1000ml at 36-38 weeks .

High volume of amniotic fluid i.e. more than 2000 ml is called

Low volume of amniotic fluid i.e. less than 400 ml is called

Polyhydramnios. It results when the fetus does not swallow the usual amount of amniotic fluid e.g. in esophageal atresiaOligohydramnios. Renal agenesis (failure of kidney formation) is the main cause of oligohydramnios

Oligohydramnios.; It can develop at any stage in pregnancy , although it is more common in the last trimester especially in those women whose pregnancy extends beyond the 40 weeks since the amniotic fluid tends to decrease at that time.

Causes:

- it can also be caused by certain maternal conditions such as DM , high blood pressure.

Renal agenesis (failure of fetal kidney formation) is the main cause of oligohydramnios ,

　

                       Oligohydramnios.

The problems associated with oligohydramnios differ depending on the stage of the pregnancy.

Oligohydramnios is more likely to have serious consequences if it occurs in the first half of pregnancy than if it occurs in the last trimester. These consequences include :

Birth defects(too little amniotic fluid early in pregnancy can lead to compression of 　fetal organs, resulting in lung and limb defects)

Miscarriage

Premature birth

Still birth (due to compression of the umbilical cord).

- It is clear pale yellow fluid.